Results
| PMID | 22277765 |
| Gene Name | SGPP2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 47 |
| Population details | 47 (15 disease-free women, 16 eutopic endometrium of endometriosis-affected women, 16 ectopic endometrium of endometriosis-affected women) |
| Sex | Female |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2012 Apr;97(4):904-11. doi: 10.1016/j.fertnstert.2011.12.051. Epub Santulli, Pietro| Marcellin, Louis| Noel, Jean-Christophe| Borghese, Bruno| Fayt, Isabelle| Vaiman, Daniel| Chapron, Charles| Mehats, Celine Universite Paris Descartes, Sorbonne Paris Cite, Faculte de Medecine, AP- HP, Groupe Hospitalier Universitaire Ouest, Centre Hospitalier Universitaire Cochin Saint Vincent de Paul, Department of Gynecology Obstetrics II and Reproductive Medicine, Paris OBJECTIVE: To investigate key genes expression of the sphingosine-1-phosphate pathway in endometriotic tissues. DESIGN: A case-control laboratory study. SETTING: Tertiary care university hospital. PATIENT(S): A total of 31 women, with (n = 16) and without (n = 15) endometriosis took part in the study. INTERVENTION(S): After surgical excision with pathological analysis, endometrial specimens were obtained from women affected or not by endometriosis. MAIN OUTCOME MEASURE(S): SPHK1-2, SGPP1-2, SGPL1, SPHKAP, and S1PR1-5 messenger RNA expression by quantitative real-time polymerase chain reaction (PCR) in the endometrium of 15 disease-free women, 16 eutopic and 16 ectopic endometrium of endometriosis-affected women. The S1PR1 and S1PR2 expression were further investigated by immunohistochemistry. RESULT(S): The SGPP2 expression was decreased in eutopic and ectopic endometrium of endometriosis-affected women (1.7- and 16.7-fold, respectively). The SGPP1, weakly expressed in healthy endometrium, is up-regulated in endometriosis-affected women (11.9- and 64.7-fold, respectively), but its expression remains low. The SGPL1 expression was decreased in ectopic endometrium (3.3-fold) and SPHKAP expression was increased in ectopic endometrium (112.6-fold) compared with endometrium of disease-free women. In endometriosis-affected women, S1PR3 expression was decreased in eutopic and ectopic endometrium (2.1- and 6.3-fold, respectively); S1PR2 and S1PR1 expression was increased in eutopic (2.5-fold) and ectopic endometrium (2.6-fold). These increases were confirmed at the protein levels by immunohistochemistry. CONCLUSION(S): Expression of the enzymes implicated in the regulation of the sphingosine-1-phosphate level balance and of its receptors is overall heavily deregulated in endometriotic lesions in favor of a decreased sphingosine-1-phosphate catabolism. Our results plead for a role of the sphingosine pathway in establishing and survival of endometriotic lesions. Mesh Terms: Aldehyde-Lyases/genetics| Analysis of Variance| Case-Control Studies| Endometriosis/genetics/*metabolism| Endometrium/*chemistry| Female| Hospitals, University| Humans| Immunohistochemistry| Lysophospholipids/*metabolism| Membrane Proteins/genet |
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